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KMID : 0613820090190070905
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Journal of Life Science
2009 Volume.19 No. 7 p.905 ~ p.916
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Microarray Analysis of Gene Expression Affected by Water-extracts of Pinelliae rhizoma in a Hypoxic Model of Cultured Rat Cortical Cells
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Kwon Gun-Rok
Jung Hyun-Jung
Shin Gil-Cho
Moon Il-Soo
Lee Won-Chul
Jeong Seung-Hyun
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Abstract
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Pinelliae rhizoma (Pr, 半夏) is a traditional medicine used in the treatment of incipient stroke. We investigated the effects of Pr on gene expression in a hypoxic model using cultured rat cortical cells. Pr (2.5 ㎍/㎖) was added to the culture medium on DIV 12. A hypoxic shock (2% O₂/5% CO₂, 37℃, 3 hr) was given two days later (on DIV 14), and total mRNAs were isolated at 24 hr post-shock from both Pr-treated samples and untreated control cultures. Microarray using TwinChip™ Rat-5K (Digital Genomics, Seoul) indicated that Pr upregulated genes for cell growth and differentiation (tubb5, tgf α, ptpn11, n-ras, pdgfa) and antiapoptosis (mcl-1), while downregulating the apoptosis-induced gene (tieg). Therefore, it is interpreted that Pr protects neurons from hypxoic shock by maintaining cell growth and differentiation and by preventing apoptosis.
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KEYWORD
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Cortical cell culture, hypoxia, microarray, Pinelliae rhizoma
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